How does it work?

Structured Illumination Microscopy

Podocyte foot processes have a size of only 150 nm. Until recently these cells could only be visualized using the electron microscopy. Now with the aid of the super-resolution microscopy it is possible to make the podocytes foot processes and the filtration slit visible under a fluorescence light microscope.

Kidney sample of a mice with Widefield Microscopy compared to SIM

PEMP

Kidney slices are stained with an antibody (Nephrin) that makes the filtration slit visible. The filtration slit resides between the podocytes.

PEMP calculates a single value for a slice – Filtration Slit Density (FSD). This value describes the ratio between the length of the filtration slit and the surface area.

Simplified a high value describes an intact morphology whereas a low value shows an effacement.

Terminate-and-forget.

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