Quantification of
podocyte foot process
morphology.

P  odocyte

E  xact Morphology

easurement

P  rocedure

PEMP is a quantitative and quick procedure measuring the slit membrane density. It is ideal to determine the exact foot process morphology in biopsies and kidney sections of mice and rats. PEMP is currently for research use only.

Benefits of PEMP

  • Single, objective & comparable value

  • Measurement per Glomerulus

  • Identification of small changes

  • High accuracy – Analysis of 20 Glomeruli per microscopic slice

  • Staining with up to 3 antibodies (WT1, Synaptopodin, etc.)

In 80% of kidney diseases podocytes are altered.*

Quantification

Get a single value describing the podocyte morphology of the sample. PEMP identifies even small changes in the morphology. 

Precision

PEMP analysis of 20 Glomeruli (or even more) per section. This ensures a comprehensive evaluation with statistical inference.

Biomarkers

Up to 4 different biomarkers can be used per slice, allowing a qualitative analysis of the sample. You can choose from biomarkers from our existing pool or send us your preferred biomarker.

3D Reconstruction

3D reconstruction of the podocyte morphology including depth color coding.

PEMP in Peer-Reviewed Research

Structured illumination microscopy and automatized image processing as a rapid diagnostic tool for podocyte effacement.
Siegerist F, Ribback S, Dombrowski F, Amann K, Zimmermann U, Endlich K, Endlich N
Scientific Reports (2017) PMID 28904359

ARP3 controls the podocyte architecture at the kidney filtration barrie.
Schell C, Sabass B, Helmstaedter M, Geist F, Abed A, Yasuda-Yamahara M, Siglek A, Maier JI, Grahammer F, Siegerist F, Artelt N, Endlich N, Kerjaschki D, Arnold HH, Dengjel J, Rogg M, Huber TB
Developmental Cell (2018) PMID 30503751

Comparative analysis of podocyte foot process morphology in three species by 3D super-resolution microscopy
Artelt N, Siegerist F, Ritter AM, Grisk O, Schlüter R, Endlich K, Endlich N
Frontiers in Medicine (2018) PMID 30425988

Novel Microscopic Techniques for Podocyte Research
Siegerist F, Endlich K, Endlich N
Frontiers Endocrinol (2018) PMID 30050501

The Role of Palladin in Podocytes
Artelt N, Ludwig TA, Rogge H, Kavvadas P, Siegerist F, Blumenthal A, van den Brandt J, Otey CA, Bang ML, Amann K, Chadjichristos CE, Chatziantoniou C, Endlich K, Endlich N
Journal of the American Society of Nephrology (2018) PMID 29720549

Cosmc-dependent Mucin-Type O-linked Glycosylation Is Essential for Podocyte Function
Stotter BR, Talbot BE, Capen DE, Artelt N, Zeng J, Matsumoto Y, Endlich N, Cummings RD, Schlondorff JS
Am J Physiol Renal Physiol (2020) PMID 31904283